Identification of peptides interfering with the LRRK2/PP1 interaction
Fig 5
Concentration and time-dependent internalization of FITC-labelled Mut3DPT-LRRK2-Short and Mut3DPT-LRRK2-Long peptides.
A) MDA-MB231 cells were incubated 4h with different concentrations of FITC-labelled peptides. The mean fluorescence intensity was detected by flow cytometry (FACS) and compared to non-treated control cells. The experiment was repeated two times with triplicates. Standard deviation is shown, as well as p values. B) MDA-MB231 cells were incubated with 20 μM of the FITC-labelled peptides for different periods of time. The mean fluorescence intensity was detected as above. Non-treated cells were used as control. Bars indicate standard deviation. Data are representative of two experiments with triplicate samples. SD is shown, as well as p values. A representative plot histogram of flow cytometry of control cells, cells treated with 50 μM of peptide or cells treated for 4h with the peptide is shown.