Bach1 promotes muscle regeneration through repressing Smad-mediated inhibition of myoblast differentiation
Fig 8
Overexpression of Bach1 does not affect differentiation of C2C12 cells.
(A) Western blotting of control and Bach1-overexpressing C2C12 cells before (day 0) and after inducing differentiation (day 2). (B) Immunohistochemical staining of MHC and nuclei (DAPI) of control and Bach1-overexpressing C2C12 cells 5 days after inducing differentiation. (C) Fusion index after 5 days. (n = 3).