An optimized method for plasma extracellular vesicles isolation to exclude the copresence of biological drugs and plasma proteins which impairs their biological characterization
Fig 4
pEV isolation by extended SEC column shows less protein impurities.
From 500μl pfp obtained from 6 RA patients pEVs were isolated by SEC or eSEC and particle concentration was measured in eluted fractions 3–7 by NTA (A). Compared SEC and eSEC size-profile from 1 representative pEV sample (fraction 5) is shown (B). Particle concentration of SEC or eSEC isolate pEVs in fraction 5 (n = 6) were measured by NTA (C). Protein content was measured by micro-BCA protein assay of each SEC or eSEC isolated pEV sample (D) and own IgG levels were measured by ELISA (E). Statistically significant differences in protein content were determined by paired T-test, *** p<0.001.