Over-expression of miR-223 induces M2 macrophage through glycolysis alteration and attenuates LPS-induced sepsis mouse model, the cell-based therapy in sepsis
Fig 5
Over-expression of miR-223 in LPS-activated macrophages reduces HIF-1α expression, while miR-223 over-expression in IL-4-activated macrophages decreases expression of PDK-1 and PFK.
The diagram demonstrates enzymes that associated with glycolysis pathway (A) and the gene expression of these enzymes in macrophages with the over-expression of miR-223 or control mimic (Ctl mimic) after the stimulation with LPS or IL-4 (B-F) are demonstrated. HIF-1α, hypoxia-inducible factor-1α; SLC2a10, solute carrier family 2,facilitated glucose transporter member 10; PFK, phosphofructokinase; LDHA, lactate dehydrogenase A; PDK1, pyruvate dehydrogenase kinase 1, PDH: pyruvate dehydrogenase; Acetyl-CoA, Acetyl Coenzyme A; TCA cycle, tri-carboxylic acid cycle or Krebs cycle.