Intracellular Cryptococcus neoformans disrupts the transcriptome profile of M1- and M2-polarized host macrophages
Fig 2
Expression of iNos is increased in Cn-infected M1-polarized macrophages.
(A) Schematic to represent macrophage infection protocol used for subsequent western blotting and transcriptome profiling experiments. (B) Western blot analysis of RAW264.7 macrophages for M1 (iNos) and M2 (Arg-1) markers after the indicated treatments. (C) iNos and (D) Arg-1 levels in (B) were quantified by densitometry based on six discrete biological repeats. Error is represented as S.E. Statistical differences between samples were appraised by one-way ANOVA followed by a Tukey’s multiple comparison test. Statistical significance is indicated as follows: **, p < 0.01.