Novel recombinant Mce-truncated protein based ELISA for the diagnosis of Mycobacterium avium subsp. paratuberculosis infection in domestic livestock
Fig 1
Characterization of sub-cultured of MAP reference strain (S5) on HEY medium.
(A) Characterization of sub-cultured MAP ‘S 5’ colonies (B) Microscopic view of acid fast bacilli (indistinguishable to MAP) under oil immersion (100X). Molecular Characterization of MAP ‘S5’ DNA: (C) IS900 PCR: Lane M- 100 base Marker (#SM0243, Fermentas), Lane 2: IS900 PCR product; (D) IS1311 PCR and IS1311-REA genotyping: Lane M: 100 base Marker (#SM0243, Fermentas), Lane 1: IS1311 PCR product, Lane 2: Digested PCR product of IS1311 from Positive control, Lane 3: Digested PCR product of IS1311 from sub-cultured colonies.