Naringenin mitigates autoimmune features in lupus-prone mice by modulation of T-cell subsets and cytokines profile
Fig 6
Naringenin increased the percentage of Tregs in MRL/MpJ-Faslpr/J mice.
Twelve-week-old MRL/MpJ-Faslpr/J mice were orally administered with Naringenin at 100 mg/kg every day for six weeks. Total splenic cells were stained with anti-CD3-BV421, anti-CD4-APC, anti-CD25-APC-Cy7 and anti-FoxP3-Alexa488 as described in Materials and methods. Subsequently the cells were analyzed by flow cytometry. (A) Naringenin oral administration increased the percentage of Tregs compared with vehicle. Representative dot plots from MRL/MpJ-Faslpr/J mice under the indicated treatments. (B) Percentage of CD4+CD25+FoxP3 Tregs. Statistical analysis was performed using one-way ANOVA by Tukey´s test. Data are presented as mean ± SD, n = 5. ***p≤ 0.001 compared versus vehicle.