Evaluation of canonical Hedgehog signaling pathway inhibition in canine osteosarcoma
Fig 5
Caspase assay and caspase Western blot reveal apoptosis in canine OSA cell lines treated with vismodegib.
Increased cleaved caspase 3 & 7 protein expression was detected with vismodegib treatments at the respectively determined IC50 value in Abrams and D-17 canine OSA cell lines when compared to vehicle control (DMSO) treatment in both (A) colorimetric caspase 3/7 assay and (B) Western blot. * p < 0.05.