Multi-kinase control of environmental stress responsive transcription
Fig 2
Identification of kinase mutants that alter environment-specific gene expression.
A) Genes differentially expressed in tunicamycin relative to YPD in wild type cells plotted for each inhibited kinase. The x-axis is the log2 fold change for the wild type in tunicamycin; the y-axis is the log2 fold change for the inhibited kinase strain relative to wild type. B) the same as (A), but for the NaCl. C) Slopes of regression lines fitted to plots like those in (A) and (B) for all inhibited kinases in all environments (missing samples in gray). D) Expression level following heat shock of the 42 genes controlled by Hsf1. Violin plots show the log2 fold change of the 42 genes in heat shock relative to wild type in heat shock for the set of inhibited kinases. Distributions of expression levels were analyzed pairwise by two-way ANOVA, revealing that Cdc15-as and Snf1-as show statistically significant attenuation of the Hsf1 operon in heat shock.