Comparison of commercially available media for hepatic differentiation and hepatocyte maintenance
Fig 4
Functional analysis of human iPS-HLCs maintained with various culture media.
Human iPS cells were differentiated into HLCs according to the protocol described in Fig 3A. Then, human iPS-HLCs were cultured with various culture media for 10 days. (A, B) The ALB (A) and urea (B) secretion capacities in human iPS-HLCs were examined. (C) The CYP1A2, CYP2C9, CYP2C19, and CYP3A4 activities in human iPS-HLCs were examined using LC-MS/MS. Results are shown as the mean ± SD (n = 3). Statistical significance was evaluated by one-way ANOVA followed by Dunnet’s test post-hoc tests (compared with “HCM” group). *p < 0.05, **p < 0.01.