Corticotropin releasing factor-overexpressing mouse is a model of chronic stress-induced muscle atrophy
Fig 4
Reduced protein synthesis and stimulated breakdown signaling were observed in CRF-OE mice.
(a) qRT–PCR analysis of relative 11beta-HSD1 mRNA expression. (b) Western blot analysis of glucocorticoid receptor (GR) phosphorylation. (c) Plasma IGF-1 concentration. mRNA and protein expression related to muscle protein synthesis were examined by (d) qRT–PCR and (e) western blot analysis, respectively. mRNA and protein expression related to muscle protein breakdown were examined by (f) qRT–PCR and (g) western blot analysis, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to WT mice. Values represent the mean ± s.e.m. of three experiments. In each experiment, sample was pooled from 5–6 mice in each group.