Methyl t-butyl ether-degrading bacteria for bioremediation and biocontrol purposes
Fig 5
Effect of treatments with Mycolicibacterium mucogenicum R8i (R8i) on tomato leaves cv. Marmande infected with Botrytis cinerea.
For induced systemic resistance (ISR) test, the true-leaf stage of the first stand growth of plants was sprayed with R8i (1x107 CFU/ml) and the second stand growth was infected with Botrytis cinerea (1x106 conidia/ml). Plants were bagged and incubated at 18°C. Disease incidence was evaluated at 48, 72 and 96 hours, measuring the necrotic area per leaf (mm2). The experiment was repeated twice and each treatment was replicated four times. Means were compared using Student Newman Keuls multiple comparison test at P < 0.05. Different letters indicate significant difference between treatments at 48, 72 or 96 hours (P < 0.05).