Elucidating the mechanism of action of alpha-1-antitrypsin using retinal pigment epithelium cells exposed to high glucose. Potential use in diabetic retinopathy
Fig 3
NFκB pathway under high glucose and with A1AT treatment.
Expression of different intermediates of NFκB pathway was measured by western blot (A) Transglutaminase 2, (B) NFκB p65, (C) TNF-α, (D) Akt, (E) pAkt, (F) levels of pAkt relativized to Akt, (G) FGFR1. Results were expressed as protein vs. Actin expression mean ± SD. *p< 0.05, **p< 0.01, ***p< 0.001, ****p< 0.0001 vs. Control. Due to the different molecular weight of Akt, TNF-α and Actin, the same PVDF membranes were incubated with the different antibodies, for this reason, Actin band pattern is repeated between Akt and TNF-α. In pAkt/Akt ratio Akt Actin was choosen to display as an example, pAkt was relativized to its actin and the same procedure was done to Akt, then pAkt/Actin was relativized to Akt/Actin. The actin and Akt bands in (E) and (F) are almost identical.