GLIPR1 expression is reduced in multiple myeloma but is not a tumour suppressor in mice
Fig 2
GLIPR1 overexpression does not affect HMCL proliferation in vitro.
(A) The expression levels of GLIPR1 mRNA in six HMCLs were assessed by RT-qPCR (normalised to ACTB). Graph depicts the mean + SD of triplicates. (B) RT-qPCR for GLIPR1 mRNA was performed on RNA from H929-EV cells and H929-GLIPR1 cells. GLIPR1 expression levels were normalised to ACTB and were expressed relative to H929-EV cells. Graph depicts the mean + SD of triplicates. ****P < 0.0001, unpaired t test. (C) The expression of GLIPR1 protein in H929-EV cells and H929-GLIPR1 cells was assessed by Western blot. HSP90 was used as the loading control. (D) The basal proliferation of H929-EV and H929-GLIPR1 cells was assessed over 3 days by WST-1 assay. Graph depicts the mean ± SD of n = 3 independent experiments.