Improved cell-specificity of adeno-associated viral vectors for medullary thyroid carcinoma using calcitonin gene regulatory elements
Fig 8
Quantification of dose- and duration-dependent improvement in transduction efficiency by rAAV-GFP in TT cell xenograft tumor.
GFP-expressing cells, detected in fluorescence micrographs of tumor sections, were counted automatically using Image J, and reported as a percentage of DAPI nuclei. Images from uninfected (Un) tumor sections, and vector-infected tumor sections (A-F) were analyzed. Tumor treatments A-F varied in vector, dose, and infection duration. P-values < 0.05 were considered significant, as determined by T-test for pairwise comparison of treatments, following ANOVA, and are indicated by asterisks. Circled asterisks indicate p-values that were Bonferroni corrected for multiple comparisons. Corrected p-values (pc) < 0.05, were considered significant for the indicated pairwise comparisons between treatments. The number of tumors (N) per treatment are shown in bolded integers.