Point-of-care diagnostic (POCD) method for detecting Bursaphelenchus xylophilus in pinewood using recombinase polymerase amplification (RPA) with the portable optical isothermal device (POID)
Fig 6
Detection of environmental DNA (eDNA) from B. xylophilus in pinewood logs.
a) Summary data of the absorbance values of the RPA assay measured using pinewood logs which were incubated at 50°C and 40% humidity. The blue dashed line and the grey background represents the regression model line and 95% confidence interval, respectively. b) Evaluation of the RPA assay for the remaining gDNA from B. xylophilus in pine trees placed in natural conditions. The RPA assay was conducted once a month for four months and the number of B. xylophilus per 10 g of pinewood for each log was assessed for every RPA assay. Black dashed lines and colored circles in each panel represent offset values and each log, respectively.