Serum amyloid P component promotes formation of distinct aggregated lysozyme morphologies and reduces toxicity in Drosophila flies expressing F57I lysozyme
Fig 6
The effect of SAP on in vitro fibril formation of I59T lysozyme.
I59T lysozyme (3.4 μM) alone (black bars), I59T with SAP (1:1 molar ratio; grey bars) and SAP alone (3.4 μM; dark grey bars) were incubated at 60°C with stirring and time points were analysed for ThT fluorescence emission (480 nm), in duplicate (A). ThT emission spectra for ThT alone (black), SAP alone (grey), I59T alone (blue) and I59T with SAP (red), analysed in triplicate (B). Representative TEM images for the aggregation samples (C). Scale bar is 100 nm.