Use of Nanotrap particles for the capture and enrichment of Zika, chikungunya and dengue viruses in urine
Fig 3
NT particles preserves ZIKV infectivity.
106 PFU/mL of (A) ZIKV and (B) CHIKV were spiked in human saliva with or without CN1030 incubation in 1:10 ratio (CN1030: saliva-spiked virus) for 30 min at ambient temperature. Viral titers were subsequently determined by plaque assays at 0, 24, 48, and 72 hours post-incubation at 37°C. Inserted images in panel B show the plaque morphology and sizes of CHIKV. ND, not detected. Data were presented as mean ± SEM of at least three biological replicates and analyzed with two-way ANOVA (***, P<0.001).