Leucine zipper transcription factor-like 1 binds adaptor protein complex-1 and 2 and participates in trafficking of transferrin receptor 1
Fig 3
LZTFL1 and AP-1 colocalize at the TGN.
(A) HeLa cells were incubated with or without 7.5 μg/ml BFA for 2 minutes, washed with complete DMEM to remove BFA, and incubated with complete DMEM for 0, 15, 30, and 60 minutes. Cells were fixed and stained with antibodies to LZTFL1 (green), γ subunit of AP-1 (red) and p230 (TGN marker, magenta). Scale bar = 10 μm. (B) Quantitative analysis of the colocalization of LZTFL1 and AP-1 at the TGN (mean + SD). Student’s t-test, n>30 cells, ***p<0.001.