Leucine zipper transcription factor-like 1 binds adaptor protein complex-1 and 2 and participates in trafficking of transferrin receptor 1
Fig 2
DFLR (DxxFxxLxxxR) motif of LZTFL1 is essential for binding to AP-1 and AP-2 complexes.
(A) Schematic representation of wild-type and LZTFL1 mutants. (B) Cell lysate from HEK293FT cells expressing FLAG-tagged wild-type LZTFL1 or the indicated mutants was analyzed by western blotting using anti-FLAG, anti-γ subunit of AP-1, anti-β1 subunit of AP-1, and anti-β-actin antibodies. Cell lysate was immunoprecipitated with anti-FLAG M2 magnetic beads. The interacting proteins were eluted by 3X FLAG peptide and analyzed by western blotting using anti-FLAG, anti-γ subunit of AP-1, and anti-β1 subunit of AP-1 antibodies. (C) Lysate from HEK293FT cells transiently expressing GFP-FLAG, LZTFL1-FLAG, or the indicated mutants was analyzed by western blotting using anti-FLAG, anti-α subunit of AP-2, and anti-tubulin antibodies. Lysate was immunoprecipitated and analyzed by western blotting using anti-FLAG and anti-α subunit of AP-2 antibodies.