Chemogenomic profiling to understand the antifungal action of a bioactive aurone compound
Fig 8
Flow-cytometric analysis of the effects of aurone SH1009 on cell cycle progression in C. albicans SC5314.
DNA histogram plots showing the percentages of cells in G0/G1 phase (pink peak on left), S phase (green center peak) and G2/M phase (blue peak on right) as a percentage of the total count of cells that were stained with Propidium Iodide (red fluorescence) and analyzed using the Millipore Guava flow cytometer and the Guava PCA-96 software system. A) Untreated cells at 0 time, B) untreated cells after 3 h of additional incubation, C) aurone SH1009-treated cells after 3 h of treatment with IC50 SH1009 concentration (16 μM), and D) CytoD-treated cells after 3 h of treatment (25 μM) as a positive control. E) The significance comparison, P values (**** P ≤ 0.0001), (***P ≤ 0.001), (**P ≤ 0.01) from a two-way ANOVA used to compare three population groups of G0/G1, S, and G2/M phases between untreated cells and SH1009-treated cells.