The polarity protein Dlg5 regulates collective cell migration during Drosophila oogenesis
Fig 8
Ability of various truncated forms of Dlg5 to rescue the BC migration delay in dlg5EP2087 mutant mosaic clones.
A-I, Confocal images showing different Dlg5 truncation forms rescuing BC migration delay in dlg5EP2087 mutant mosaic clones. The full-length (FL) (A, B) and Δ2 (E) fully rescued the BC migration delay. Whereas C4, Δ1, Δ5, Δ6, Δ7 and Δ8 had no or little rescue ability (C, D, F-I). Mutant clones were marked by the absence of the GFP or RFP (green). Transgenes and aPKC were marked by red and white respectively. J-K’, Escapers from dlg5KG748 homozygous rescued by C4 showed completely no migration of BC clusters and misdistribution of Ecad-GFP (green, K) and reduction of aPKC (white, K’) compared to the wild-type Ecad-GFP (J, J’). L, Quantification of BC migration delay in different genotypes. EP2087, dlg5EP2087. DAPI was marked by blue. Scale bars: 20μm.