The polarity protein Dlg5 regulates collective cell migration during Drosophila oogenesis
Fig 4
Subcellular localization of Dlg5 in BC clusters identified by Ubi-Dlg5.EGFP or Ubi-Dlg5.TagRFP-T.
A-D’, Subcellular localization of Dlg5-GFP (green) expressed by Ubi-Dlg5.EGFP in BC clusters. Dlg5-GFP is colocalized with Dlg5-TagRFP-T expressed by the genomic construct Dlg5-TagRFP-T in surfaces between BCs and PCs (A). Dlg5-GFP is colocalized with Dlg (D), partially colocalized with Arm (C) in surfaces between BCs and PCs, but not colocalize with Sqh-mCherry in surfaces between BCs and nurse cells (B). At apical domain of BC clusters, a small region of overlap between Dlg5-GFP (green) and aPKC (blue) was detected (C’, D’). Arrowheads indicate the apical overlap between Dlg5-GFP and aPKC (C’, D’). E-F’, High resolution confocal images obtained using a Zeiss LSM 880 with Airyscan super-resolution mode. Arrows indicate the partial colocalization between Dlg5-RFP and Arm in BC clusters (E-F’). G-H’, Subcellular localization of Dlg5-RFP (red) expressed by Ubi-Dlg5.TagRFP-T in BC clusters. White dotted circles indicated the BC cluster in G. Dlg5-RFP mainly localized in surfaces between BCs and PCs (G-H’). BC cluster was marked by GalT-GFP (H, H’). Z-series projections (H) and three-dimensional confocal reconstruction (H’) of the BC cluster were shown. The spot-like aggregations of Dlg5-RFP between BCs and nurse cells were not localized in BC clusters (H’). Blue color represents DAPI in A, B and G but represents aPKC in C’ and D’. PCs were indicated by asterisks. Scale bars: 20μm in G and 10μm in others.