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The polarity protein Dlg5 regulates collective cell migration during Drosophila oogenesis

Fig 2

The apical-basal polarity of BC clusters.

A-F, Single confocal sections (A, C, E) and corresponding z-series projections (B, D, F) of BC clusters at early stage 9 (A, B) and middle stage 9 (C, F) egg chambers. Side views were shown in A-D. Basolateral views were shown in E. Apical views of z-series projections were shown in F. G and H, Two single confocal sections of the same BC cluster at middle stage 9. Both apical views (G) and basolateral views (H) were shown. I and J, Side views of the BC cluster at late stage 9. Both single sections (I) and z-series projections were shown (J). K and L, Single sections of BC clusters at stage 10. M, Schematics of apical-basal polarity proteins distribution in BC clusters. Side view (left), basolateral view (middle) and apical view (right) were shown. sing., single sections. proj., z-series projections. bc, border cell. pc, polar cell. PCs were indicated by asterisks. Green represents Nrg-GFP from A to K but represents Ecad-GFP in L. Red represents Arm and blue represents aPKC. Scale bars: 10μm.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0226061.g002