Investigating gene expression profiles of whole blood and peripheral blood mononuclear cells using multiple collection and processing methods
Fig 3
Two dimensional Sammon projection using the top 50% of most variable transcripts (25370) for whole blood (red) and PBMC (black). There is a clear distinction in clusters for PBMCs and whole blood sample processing as marked by a black tilted line. All whole blood samples are shown on left side and PBMC samples are on the right side of the plot. The (F) and (C) denotes the frozen and cold conditions for the sample collection and storage. The description of all the legends is given here. PBMCs samples: ACD_Magnetic- blood collected in ACD-A tubes and separated using magnetic method followed by extraction using TRIzol® method. CPT—blood collected in CPT tubes and PBMCs separation using manufacturer recommendations followed by extraction using TRIzol® method. EDTA_LSM- blood collected in EDTA tube and PBMC separation using LSM followed by extraction using TRIzol® method. EDTA_Leukolock- blood collected in EDTA tube, PBMCs separated using LeukoLOCK™ method followed by extraction using TRIzol® method. Whole blood samples: ACD- blood collected in ACD-A tube followed by RNA extraction using TRIzol® LS method. ACD_RBC lysis- blood collected in ACD-A tube followed by RBC lysis using ACK buffer and RNA extraction using miRNAeasy extraction kit. EDTA_RBC lysis -blood collected in EDTA tube followed by RBC lysis using ACK buffer and RNA extraction using miRNAeasy extraction kit. EDTA- blood collected in EDTA tube followed by RNA extraction using TRIzol® LS method. PAXgene- blood collected in PAXgene® tube followed by RNA extraction using PAXgene® blood miRNA kit. RNAgard—blood collected in RNAgard® tube followed by RNA extraction using Biomaxi™ Precip Buffer and PAXgene® Blood miRNA kit.