Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis
Fig 2
Subcellular localization and transcriptional activation analysis of the AmDREB3 protein.
The pBI-AmDREB3:GFP fusion structure and control plasmid pBI-GFP were separately transferred into Arabidopsis leaf protoplasts using a polyethylene glycol-mediated method. (A) The images under dark, bright, and merged fields are presented. (B) Growth of the transformants with pGBKT7-AmDREB3, pGBKT7, or Y2HGold (the latter two were used as negative controls) on SD/-Trp or SD/-His medium and the blue color in the X-α-Gal assay are shown.