Allele-specific enhancer interaction at the Peg3 imprinted domain
Fig 4
DNA methylation levels of several ECRs localized upstream of Peg3.
The schematic diagram shows the relative positions of 19 ECRs (Evolutionarily Conserved Regions) within the mouse Peg3 domain (upper panel). The neonatal brain of F1 mice derived from the crossing of C57BL/6J (B6) females and PWD/PhJ (PWD) males were used for isolating genomic DNA, which were then used for the analyses of DNA methylation levels. The DNA methylation levels of six individual ECRs were summarized and presented with open and closed circles indicating unmethylated and methylated CpG sites (lower panel). The methylation levels of each allele for a given ECR were calculated and presented. In the case of ECR19, a presumable SNP was not found from the F1 hybrid that had been used for the current study, thus the results were presented without allele sorting.