Novel chemotherapeutic agent, FND-4b, activates AMPK and inhibits colorectal cancer cell proliferation
Fig 3
Cell proliferation of commercial CRC cell lines treatment combinations.
(A) Western blot analysis of commercially-available CRC cell lines treated with 10μM FND-4b, 5μM PI-103, and 100nM SN-38, alone and in combination versus untreated control (media alone) for 24h duration. β-actin was used as a loading control. The images are representative of three independent experiments. In all CRC cell lines, FND-4b mono- and dual-treatment resulted in decreased cyclin D1 expression and—with the exception of DLD1—increased pAKT expression compared to untreated control. (B) SRB Cytotoxicity Assays of commercially-available CRC cell lines treated with 10μM FND-4b, 5μM PI-103, and 100nM SN-38, alone and in combination for 48h duration. Graphic representations are the mean ± SD plotted as a percentage relative to untreated control; each measurement was performed with 5 replicates. *p<0.0001 vs. control and #p<0.001 vs. FND-4b alone.