TEM8/ANTXR1-specific CAR T cells mediate toxicity in vivo
Fig 2
CAR T cell responses to mouse and human TEM8.
Human T cells engineered to express TEM8-specific CARs (L1, L2, L3, L5, 1D2) or mock-transduced T cell controls were tested for response to LS174T cells engineered to express full-length mouse TEM8. Antigen specific responses were detected using (A) IFN-gamma ELISA and (B) cytotoxicity assay (tested at effector:target (E:T) ratios of 10:1 and 5:1). (C) Responses to titrated concentrations of recombinant human and mouse TEM8 protein were assessed by IFN gamma release. In all cases CAR-T cell lines were diluted with mock T cells to equalise for transduction efficiency. Graphs show the mean of triplicate cultures (± standard deviation, SD) and are each representative of 3 experiments.