Adaptation of H3N2 canine influenza virus to feline cell culture
Fig 3
pH range of syncytium formation of the mutant HAs in MDCK cells.
MDCK cells were co-transfected with a mutant (HA1-K299R or HA2-T107I) or wild-type (HA-WT) HA expression plasmid and a Venus expression plasmid. After treatment with the indicated pH buffers, the cells were observed under a fluorescence microscope. The red lines indicate the boundaries of pH at which cell fusion occurred. The assay was conducted three times independently and the representative data are shown.