Irisin promotes C2C12 myoblast proliferation via ERK-dependent CCL7 upregulation
Fig 3
Transcriptomic analysis of irisin-regulated gene expression in C2C12 cells.
(A) Gene ontology (GO) analysis of irisin-regulated transcriptome in C2C12 cells. The graph shows–log p values (modified Fisher’s exact P-values) obtained from GO analysis in terms of ‘biological processes’ using microarray data [fold change ≥ 2, pooled samples (n = 3) per group]. (B) mRNA expression levels of enriched genes involved in positive regulation of ERK signaling pathway obtained from microarray data. (C) Validation of expression levels of genes involved in positive regulation of ERK cascade (Ccl2, Ccl7, Ccl8, C3, and Pdgfa). Relative mRNA levels were analyzed by qRT-PCR using SYBR Green dye (n = 3 per group).