BRAF V600E and Pten deletion in mice produces a histiocytic disorder with features of Langerhans cell histiocytosis
Fig 5
Histology of thymus, spleen, and testis in BrafCA and BrafCATg(CD207-cre) mice.
A. Histology of thymus, spleen, and testis. Hematoxylin and eosin staining of representative microscopic sections of thymus, spleen, and testis from BrafCA and BrafCATg(CD207-cre) mice at 40X and 200X magnification. The sharp cortico-medullary border in the thymus of BrafCA mice (arrows) is obscured in the thymus of BrafCATg(CD207-cre) 19 day old female mice. Similarly, the sharp border between red pulp and white pulp in the spleen of BrafCA mice (arrows) is obscured in the spleen of BrafCATg(CD207-cre) 36 day old male mice. Spermatozoa which are visible in the testis of 36 day old BrafCA mice are absent from the testis of 42 day old BrafCATg(CD207-cre) mice. Original magnifications, 40X and 200X as indicated. B. Androgen receptor expression in testis and epididymis. Immunohistochemical staining for androgen receptor of testis and epididymis from BrafCA and BrafCATg(CD207-cre) mice. Androgen receptor staining is apparent in Leydig and some Sertoli cells in BrafCA mice but not BrafCATg(CD207-cre) mice (both 58 days of age). However, androgen receptor staining is present in the epididymis of both genotypes at the same age. Scale bars are shown.