Transthyretin expression in the postischemic brain
Fig 4
TTR levels at different time points after PT.
(A) The ischemic territory region was sampled for protein extraction (coronal section of an adult mouse brain adapted from [21]. (B) TTR protein is absent in the ischemic territory 48 hours after PT and absent in the brain parenchyma of wild type mice not subjected to ischemic stroke. (C) TTR is not expressed in the ischemic territory at different time points after PT: 24 hours, 48 hours, 7 days and 14 days, respectively. (D) TTR protein is absent in the ischemic territory 14 days after PT and absent in the brain parenchyma of sham operated mice. Loading of 10 μg of total protein. Anti-TTR rabbit antibody diluted at 1:3000 (ThermoScientific cat# PA5-27220, Rockford, USA). CSF–cerebrospinal fluid; IC—Infarct Core; M–Marker; Neg–negative control (recombinant legumain 2 μg); PI–Peri-infarct area; MS–Positive control (mouse serum); PT–Photothrombosis; TTR–Transthyretin.