EGF receptor stimulation shifts breast cancer cell glucose metabolism toward glycolytic flux through PI3 kinase signaling
Fig 3
EGF acutely and dose-dependently activates T47D cell EGFR.
Western blots and protein-band intensities of phosphorylated EGFR (p-EGFR) after EGF treatment. Cells were treated with 100 ng/mL EGF for 30 min (left) or 5 and 60 min (middle), or with graded EGF concentrations for 5 min (right). Bars are mean ± SD of % control obtained from triplicate (left and middle) or duplicate (right) samples. *, P <0.05; †, P <0.005, compared to controls.