Elimination of activating Fcγ receptors in spontaneous autoimmune peripheral polyneuropathy model protects from neuropathic disease
Fig 4
Activating FcγRs depletion suppresses P0 specific T cell response and LPS-induced macrophage activation.
A. Images from IFN-γ ELISPOT assay showing positive spots from Fcer1g+/+ B7-2-/- and Fcer1g-/- B7-2-/- splenocytes in the presence of P0 peptide. B. Quantitative ELISPOT assay analysis showing that IFN-γ positive P0 peptide reactive cells are significantly reduced in Fcer1g-/- B7-2-/- compared to Fcer1g+/+ B7-2-/- mice (n = 7 for each genotype). C. Representative flow plots showing the differences in proportion of TNFa or IL-6 producing CD11b+ cells from Fcer1g+/+ B7-2-/- and Fcer1g-/- B7-2-/- mice with or without LPS (Cont) stimulation. D. Quantification of flow cytometry analysis showing the expression of proinflammatory cytokines in CD11b+ cells from Fcer1g+/+ B7-2-/- and Fcer1g-/- B7-2-/- mice with or without LPS (Cont) stimulation (n = 8). *P < 0.05.