Inhibitors of AKT kinase increase LDL receptor mRNA expression by two different mechanisms
Fig 1
AKT inhibitors increase LDLR levels in a dose-responsive manner.
HepG2 cells were cultured in the presence of vehicle, the indicated concentrations of AKT inhibitors or the GSK3β inhibitor, SB216763, for 14 h before harvesting for analysis by immunoblotting with antibodies against LDLR and β-tubulin. The figure shows one representative blot from four independent experiments. The bar graphs show quantification of the immunoblots which were scanned and the intensity of the LDLR band was normalized to that of β-tubulin, and plotted relative to the values obtained for vehicle-treated cells, which were set at 1. Error bars represent SD. *p < 0.5 relative to vehicle-treated cells.