Polyamine biosynthesis in Xenopus laevis: the xlAZIN2/xlODC2 gene encodes a lysine/ornithine decarboxylase
Fig 9
Influence of the C-terminal region of xlAZIN2 in the degradative process induced by AZs.
HEK293T cells were transfected with: (A) xlAZIN2, (B) xlAZIN2 lacking the 21 C-terminal residues (xlAZIN2-ΔC) or (C) with a construct coding for a chimeric protein with the substitution of the 21 C-terminal residues of xlAZIN2 by the C-terminal segment of mouse AZIN2 (xlAZIN2-mAZIN2). In parallel, each one of the constructs was co-transfected with members of the AZ family (AZ1, AZ2, and AZ3). Western blots were probed with anti-Flag antibody. On the right side, schematic representations of xlAZIN2 and the two mutated proteins.