Ectopic expression of tea MYB genes alter spatial flavonoid accumulation in alfalfa (Medicago sativa)
Fig 5
Morphology and flavonoid analyses in the flowers of CsMYB5-1/CsMYB5-2 over-expressing alfalfa plants.
(A, F) Flower phenotype of the transgenic alfalfa plants over-expressing CsMYB5-1 (A), CsMYB5-2 (F) in comparison with the wild type; (B, G) Relative anthocyanin content in flowers of the transgenic alfalfa plants over-expressing CsMYB5-1 (B) or CsMYB5-2 (G) in comparison with the wild type; (C, H) Relative total flavonoid content in flowers of the transgenic alfalfa plants over-expressing CsMYB5-1 (C) or CsMYB5-2 (H) in comparison with the wild type; (D, I) Relative soluble proanthocyanidin content in flowers of the transgenic alfalfa plants over-expressing CsMYB5-1 (D) or CsMYB5-2 (I) in comparison with the wild type; (E, J) Relative insoluble proanthocyanidin content in flowers of the transgenic alfalfa plants over-expressing CsMYB5-1 (E) or CsMYB5-2 (J) in comparison with the wild type. All the assays were performed with triplicates and the data are presented as mean ± SD, student’s t-test (n = 3, *P<0.05, **P<0.01).