Comparison of iron-reduced and iron-supplemented semisynthetic diets in T cell transfer colitis
Fig 1
Dietary iron reduction does not protect against T cell transfer colitis.
(A) Experimental design for T cell transfer colitis in Rag1-/- recipient mice fed with iron depleted (w/o Fe) or iron supplemented experimental diets (FeSO4 or Hemin). (B) Bodyweight development at indicated time points after transfer of CD4+ CD62L+ T cells to Rag1-/- mice fed with experimental diets (one of 3 independent experiments with 4–6 animals in each group is displayed; mean ± SD; one way ANOVA, not significant). (C) Clinical colitis scores at indicated time points of mice from (A) (one of 3 independent experiments with 4–6 animals in each group is displayed; mean ± SD; one way ANOVA, n.s.). (D) Representative H&E staining of colon tissues sections of mice from (A) at day 17 after T cell transfer. (E) Histological colitis scores of mice from (A) (each symbol represents one animal and horizontal lines indicate mean values for each dietary group; Kruskall-Wallis test, n.s.).