Glypican 6 is a putative biomarker for metastatic progression of cutaneous melanoma
Fig 3
GPC6 is a putative target of miR-509-3p and overexpression of miR-509-3p downregulates GPC6 mRNA levels.
(A) Predicted binding sites of miR-509-3p on the 3’-UTR of human GPC6 gene (NM005708). (B-C) A375 cells were transfected with 4nM negative control mimic (mimic ctrl) or miR-509-3p mimic for 48 hours. RNA was collected and RNA levels of miR-509-3p (B) and GPC6 (C) were measured using Taqman assays relative to Rplp0 from n = 3 experiments. Means ± S.D. **, p < 0.001; ***, p < 0.0001 for miR-509-3p mimic versus mimic control.