Fatty acids from fish or vegetable oils promote the adipogenic fate of mesenchymal stem cells derived from gilthead sea bream bone potentially through different pathways
Fig 5
Fatty acids effects on adipogenic genes expression.
Relative expression of genes related to lipid metabolism, including (A, C) enzymes (fas, lpl and hsl) and (B, D) fatty acid transporters (cd36, fatp1 and fabp11) normalized to b-actin and rps18 in gilthead sea bream bone-derived cells. Cells at day 4 were incubated with different (A, B) individual or (C, D) combined fatty acids, or were left untreated as control (dashed lines) for 6 h. Data are shown as mean + SEM (n = 3–4). Significant differences (p<0.05) among treatments are indicated by different letters. Asterisks indicate significant differences (p<0.05) with the control. EPA: eicosapentaenoic acid; DHA: docosahexaenoic acid; LA: linoleic acid; ALA: α-linolenic acid.