Molecular determinants of WNT9b responsiveness in nephron progenitor cells
Fig 5
Identification and isolation of Cited1 expressing cells from embryonic kidneys.
(A) Cryosections of E18 embryonic kidneys isolated from Cited1Cre/TomatoRed mice were assessed by immunofluorescent microscopy for the presence of TomatoRed in cap mesenchyme surrounding ureteric bud tips. (*) Ureteric Bud outlined in green. (B) Whole E15.5 embryonic kidneys were dispersed into monolayer culture in the presence of tamoxifen (2.5 μg/ml) for 16 hours and TomatoRed(+) cells were visualized by immunofluorescent microscopy. (C) Expression of WNT9b pathway component transcripts in Cited1Cre/TomatoRed cells isolated by FACS from E15.5 embryonic mouse kidney quantified by ddPCR. Bars represent mean number of events of gene of interest normalized to B2M events (n = 2, 17 total pooled embryonic kidneys). Error bars represent standard error of the mean.