The NRF2 transcriptional target NQO1 has low mRNA levels in TP53-mutated endometrial carcinomas
Fig 4
NQO1 expression in endometrial carcinoma.
NQO1 expression was measured by quantitative RT-PCR in Cochin Hospital cohort (CCH) and RNAseq in The Cancer Genome Atlas Cohort (TCGA). TCGA data was obtained from the www.cbioportal.org platform [28,29]; Uterine Corpus Endometrial Carcinoma (TCGA, Nature 2013) [2]. NS: non-significant (p>0.05); *, **, ***, ****: p-values, respectively: <0.05; <0.01; <0.001; <0.0001. Dots depicted as crosses refer to tumors considered as NRF2 activated in the Cochin cohort, as shown on Fig 3. A. NQO1 expression and histological type (CCH). Type I and type II refer to histological subtypes (Table 1). B. NQO1 expression and TP53 staining. Altered TP53 staining considered both staining with overexpression or staining with loss of expression. C. NQO1 expression and molecular group (CCH). POLE: POLE exonuclease domain mutated tumors. MSI: microsatellite instable-like tumors. TP53: TP53/copy-number high-like tumors. CNL: copy number low-like tumors. Paired comparisons computed using post-hoc test adjusted for the risk α for multiple comparisons. POLE tumors were not included in statistical test because of the number of samples in the group (<5). D. NQO1 expression and molecular group (TCGA). POLE: POLE tumors. MSI: microsatellite instable tumors. Anova: analysis of variance. p-values are depicted in * to **** for graphical purposes. Paired comparisons computed using post-hoc test adjusted for the risk α for multiple comparisons. E. NQO1 expression and histological type within copy-number high group. TCGA data. p-value based on Student t-test. F. Event free survival and NQO1 expression. Kaplan-Meier curves. p-value based on two-sided log-rank test.