miR-7977 inhibits the Hippo-YAP signaling pathway in bone marrow mesenchymal stromal cells
Fig 1
Comparative analysis of mRNA expression between control- and miR-7977-transduced MSCs.
(A) Transfection of control or miR-7977 mimic into MSCs. 5 nM of miR-7977 mimic was used for transfection. After 72 hrs, total RNA was prepared for transcriptome analysis. (B) The mRNA was analyzed by Human Gene 2.0ST Array and compared. Scattered plot demonstrates the total RNA level in control MSCs and miR-7977-transduced MSCs. (C) Cluster analysis of mRNA expression in MSCs. The gene symbols that have the target sites predicted by miRDB are indicated by red ink. CEL data were normalized by the rma method provided by Bioconductor (R commander 3.4.1). Dendrogram and heatmap were made by using the amap and gplots package. The genes shown were selected when the expression level significantly differed between control and miR-7977-transduced MSCs [control (n = 3) vs. miR-7977 (n = 3), P = 0.023]. The gene expression level in the heatmap was expressed by intensity-based Z-score.