Characterization of an intratracheal aerosol challenge model of Brucella melitensis in guinea pigs
Fig 7
Histopathology of the uterus following Brucella infection.
(A) Representative images of histopathology and immunohistochemistry of the uterus following intratracheal inoculation with PBS (top), B. melitensis 16M at low dose (middle), or high dose (bottom) at 30-days post-inoculation. (B) Sections were scored for severity from 1–4 (S1 Table) based on edema, endometrial neutrophilic inflammation, and myometrial inflammation. The white dashed box in the left panel indicates the section highlighted for higher magnification in the middle and right panels. The high dose group had increased numbers of neutrophils in the endometrium, foci of histiocytic inflammation within the myometrium (*), and Brucella antigen was detected intracellularly via IHC (arrows). Magnification 4x (left, H&E, bar = 200 μm), 20x (middle, H&E, bar = 50 μm), 40x (right, Anti-Brucella IHC, bar = 20 μm).