The knocking down of the oncoprotein Golgi phosphoprotein 3 in T98G cells of glioblastoma multiforme disrupts cell migration by affecting focal adhesion dynamics in a focal adhesion kinase-dependent manner
Fig 3
The knocking down of GOLPH3 affects directional persistence of T98G cells.
(A-E) The indicated cells were left untreated (shLuc and shGOLPH3) or subjected to lentiviral transduction for 5 days to express RNAi-resistant GOLPH3 (shGOLPH3 + LV). (A) Confluent cultures of the indicated cells grown in 35-mm glass-bottom culture dishes were wounded with a sterile tip. The dishes were transferred to a microscopy heating stage equipped with temperature, humidity and CO2 comptrollers, and phase-contrast images were acquired immediately, and every 5-min up to 24 h. Representative images acquired at the indicated times are shown. Bar, 50 μm. (B-E) The movement of cells from consecutive images of the time-lapse microscopy shown in A was manually tracked, and each trajectory (n = 100 for each cell line) was plotted with the same origin in a Cartesian coordinate system (B). The directionality ratio (C), average speed (D), and direction autocorrelation (E) parameters were obtained using DiPer software. Bar represents the mean ± standard deviation (n = 100). ** P < 0.01; *** P < 0.001.