Deregulation of LRSAM1 expression impairs the levels of TSG101, UBE2N, VPS28, MDM2 and EGFR
Fig 5
Investigation of LRSAM1 interacting molecules in LRSAM1 or TSG101 downregulated cells.
Quantification of the RNA expression levels of the interacting molecules relative to the Untransfected control cells, which was set to 100 96h after the first siRNA transfection. Values were obtained as ratios of the RNA of interest over GAPDH control. Specifically, LRSAM1 downregulation significantly decreased the levels of LRSAM1 (30% ±3.86%, p<0.0275), TSG101 (45% ±2.19%, p<0.0092), UBE2N (52%±3.05%, p<0.0158), VPS28 (47%±3.62%, p<0.0336), EGFR (49%±3.38%, p<0.0125) and MDM2 (45%±2.44%, p<0.0096) compared to the Untransfected control cells. In contrast, AP-3 (96%±2.59%, p<0.0109) showed similar expression between the LRSAM1 knocked down and Untransfected cells. TSG101 downregulation significantly decreased the levels of TSG101 (33% ±1.11%, p<0.0068) and VPS28 (55%±2.86%, p<0.0154) and also caused a small decrease of MDM2 (79%±3.37%, p<0.0217) compared to the Untransfected cells. Expression levels of LRSAM1 (96% ±4.41%, p<0.0427), UBE2N (94%±2.97%, p<0.0172), EGFR (96%±3.96%, p<0.0298) and AP-3 (99%±4.57%, p<0.0462) were similar to the Untransfected cells.