Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst
Fig 3
Relative mRNA level of different AOX isoforms in control and harpin-treated Arabidopsis thaliana suspension cells.
Arabidopsis suspension cells were treated with HrpZpto at the final concentration of 150 nM. At the indicated time points, samples were taken, and total RNA was extracted. Quantitative RT-PCR was carried out by using specific primers designed for the coding sequences of AOX1a, AOX1b, AOX1c, AOX1d, AOX2 and mitosis protein YLS8 (housekeeping gene) genes as described in Materials and methods. The gene expression of the samples collected from each cell culture before treatments, was regarded as 1. Data are expressed as means ± SD from three independent HrpZpto treatments. (Asterisk) Significant difference with respect to control (P<0.05).