Elevated cellular cholesterol in Familial Alzheimer’s presenilin 1 mutation is associated with lipid raft localization of β-amyloid precursor protein
Fig 1
Cholesterol level was higher in the CHO PS1 ΔE9 cells than in the PS1 WT cells.
(a) Free cholesterol was visualized by filipin staining from the CHO PS1 WT and ΔE9 cells. (b) Fluorescence intensities were compared between the PS1 WT (n = 11) and ΔE9 cells (n = 13). (c) Total cholesterol levels were measured from cell membrane fractions using the Amplex Red cholesterol assay kit from the PS1 WT and ΔE9 cells (n = 7). Statistical analysis was carried out using one way ANOVA: ***p<0.001.