A serum-free medium formulation efficiently supports isolation and propagation of canine adipose-derived mesenchymal stem/stromal cells
Fig 3
Identification of growth factors required for expansion of canine Ad-MSCs under serum-free conditions.
A. Photomicrographs of canine Ad-MSCs cultured in serum-free medium with or without growth factors or DMEM/FBS-H or DMEM/FBS-S or DMEM/DOG-S. B & C. Cell number and viability were determined a week after placing in serum-free medium containing indicated growth factors or DMEM/FBS-H or DMEM/FBS-S or DMEM/DOG-S. Means of two replicates for each donor are shown.